Frequently Asked Questions

Here you will find the frequent questions asked

Choice of buffers and tissue slides

What tissue slides should I use for sections processed in Retriever?

Usually Superfrost+ and .... perform rather well. To ensure safety of your most valuable material (especially tissue arrays) we sugggest using always activated slides. You can easily prepare them in your lab following the instructions in the Manual.

I used in Retriever the EDTA pH8.0 buffer and the tissues detached from the slides

This is a general problem with compatibility of tissue slides with EDTA buffer. If you have used just regular Superfrost slides, and sometimes even Superfrost + tissue may detach in the buffer. We recomment using Hybond or Superfrost Gold slides.

The tissue did not detach from the slide, but after Retriever some of the tissue areas lost morphology

Sometimes these are internal areas of the not sufficiently fixed, or improperly processed (paraffin embedding) tissues. In principle these tissues may be used. We advice to add to your buffer glycerol to 5% or 10% and re-adjust again the pH to the required for this type of buffer. This would make processing of the tissue less stringent.

I am used to Tris Buffer for antigen unmasking, would it work in Retriever?

We do not recommend using Tris buffer with Retriever.

Can I use in Retriever standard citrate (pH 6.0) and EDTA (pH 8.0) buffers

Yes, you can. 

What if tissues are fixed with other than formalin fixatives? Can they be processed in Retriever?

One of the frequently used fixatives is formalin/acetic acid fixative. Laboratories in France and Belgium, where it is frequently used in routine pathology, report that Retriever works well with these tissues, and even superior to other means of antigen unmasking when it comes to some antigens, estrogen receptor in particular.

Problems with antibodies

Do all antibodies work in IHC after Retriever?

Most of them do. If antibody works well in immunoblotting, it, as a rule works in IHC on Retriever -processed tissue. When you have a choice, look for antibodies prepared against a fusion protein (fragment) that work in blotting. For the majority of these antibodies start with buffer A or citrate buffer (pH 6.0). Usually they give no problems. Antibodies prepared against peptide epitope working in immunoblotting, also work on sections as a rule, unless the peptide epitope contains too many Lys; this may lead to epitope inactivation, an irreversible one. Use buffer A or buffer U. Most of the problems are with monoclonal antibodies prepared against native epitopes and mainly used in flow cytometry. These often require testing a variety of buffers to find an appropriate one. 

Troubleshooting Retriever

My Retriever does not work. What to do?

In an extremely unlikely case of factory fault, please look at the troubleshooting guide in the Manual. If you still cannot resolve the problem, please contact us or your distributor. Within the first year we change machine if anythig does not work.

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écupération d'antigène, 抗原检索
antigen vyhledávání,
antigen hentning,antigen herstel,
antigeeni otsing, antigeeni haku
ανάκτηση αντιγόνου
אנטיגן אחזור
antigene recupero
항원 검색
antigenas paieška
antigen gjenfinning
بازیابی آنتی ژن
مستضد استرجاع
odzyskiwanie antigen
recuperação antigênica
antigen de regăsire
антигена преузимање
antígeno de recuperación de
antijen alımı
مائجن بازیافت

paraffin immunohistochemistry
البارافين المناعية
parafín imunohistochemie
paraffin immunhistokemi
paraffine immunohistochemie
parafiini immunohistokemiallisesti
immunohistochimie paraffine
Paraffin Immunhistochemie
פרפין אימונוהיסטוכימיה
paraffina immunoistochimica
ανοσοϊστοχημεία παραφίνη
imunohistoquímica parafina
парафин иммуногистохимия
inmunohistoquímica de parafina
parafin immünhistokimya
paraffin immunohistokemi