What tissue slides should I use for sections processed in Retriever?

Usually Superfrost+ and .... perform rather well. To ensure safety of your most valuable material (especially tissue arrays) we sugggest using always activated slides. You can easily prepare them in your lab following the instructions in the Manual.

I used in Retriever the EDTA pH8.0 buffer and the tissues detached from the slides

This is a general problem with compatibility of tissue slides with EDTA buffer. If you have used just regular Superfrost slides, and sometimes even Superfrost + tissue may detach in the buffer. We recomment using Hybond or Superfrost Gold slides.

The tissue did not detach from the slide, but after Retriever some of the tissue areas lost morphology

Sometimes these are internal areas of the not sufficiently fixed, or improperly processed (paraffin embedding) tissues. In principle these tissues may be used. We advice to add to your buffer glycerol to 5% or 10% and re-adjust again the pH to the required for this type of buffer. This would make processing of the tissue less stringent.

I am used to Tris Buffer for antigen unmasking, would it work in Retriever?

We do not recommend using Tris buffer with Retriever.

Can I use in Retriever standard citrate (pH 6.0) and EDTA (pH 8.0) buffers

Yes, you can.