W is for Western (blot)

On the basis of the same technology, as our buffers for IHC applications, we offer specially developed buffers for improving sensitivity and decreasing background in Western-blot applications.

Western blot was stained with the same antibody using standard blocking procedure (skimmed milk, above) and Western block (bleow). Note the  reduction of the noise produced by the blotting membrane.

Skimmed

Milk

Western

Block

Effect of antibody diluent on detection of antigen on solid phase with 2 step detection. The primary antibody was diluted in PBS/Tween or in Antibody Diluent W. Signal to noise ratio was 3.42 without and 17.3 with antibody diluent. Note that the specific signal (dots) is equal on both pictures.

Western Block, ready-to-use

# AP0500

Specialised solution for blocking free binding sites in Western blots on PVDF, or various types of nitrocellulose membrane. Does not contain large proteins, including serum proteins, BSA, cow milk proteins; low-molecular weight blocking components of this blocking solution do not cause non-specific binding of antibodies to blocking ingredients, while effectively prevent non-specific interactions of test antibody with membrane and transferred proteins on the blot. They also do not prevent (sterically) recognition of the target proteins on the blot due to the size. The buffer is an excellent alternative to self-made skim milk solutions, more effective and improves the signal to noise ratio. Contains ProClin® 300 as preservative and has shelf life of over 12 months at 2-8oC.

Data Sheet

Antibody Diluent W, ready-to-use

# AP0501

Specifically designed for Western blot and other solid phase immunoassay, such as immune-PCR, dot blot, protein arrays, this buffer prevents non-specific binding of antibody to solid phase and proteins. As a result, using Antibody diluent may dramatically improve the signal/noise ratio and increase the sensitivity of your assay. In our tests we have seen up to 5 times improved sensitivity due to lower background staining. Contains ProClin® 300 as preservative and has shelf life of over 12 months at 2-8oC.

Data Sheet

Stripping Buffer W, ready-to-use

# AP0503

Specially designed for re-probing Western blots, detected with chemiluminiscent and other methods with non-precipitating substrate (i.e. immunofluorescent detection).This formulation effectively and gently removes from blot primary and secondary antibodies, thus allowing to re-probe it again with a new specific antibody.

Data Sheet

Washing Buffer W Tris-Based, 10x stock

# AP0502

A concentrated stock for buffer to rinse and wash the western blot between the procedures without removing blocking and other agents preventing the non-specific reactivity of immunochemicals during the detection procedure. Contains ProClin® 300 as preservative and has shelf life of over 12 months at 2-8oC.

Data Sheet

Product Ordering Codes