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A reliable, time tested solution to unmask antigen on formalin-fixed sections
Aptum Biologics Ltd.
38 Baker's Drove
Southampton
SO16 8AD
United Kingdom
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Antigen unmasking, antigen recovery on formalin fixed paraffin embedded tissue sections
Allow us to introduce the 2100 Antigen Retriever, the best epitope recovery unit for IHC on formalin-fixed sections. It is used by hundreds of research groups and small pathology labs worldwide, offering unique features not available in analogous machines.
The initial model was released in 2006, and we now present the 2015 third iteration of the Retriever, fully optimized for the use of the Universal Buffer.
In addition, we offer other components of the Retriever program designed to ensure a fully standardized and reproducible IHC process.
Excellent Epitope Recovery.
Every Time.
We express our gratitude to all the users of the Retriever for such a high rating!
The award-winning search engine, BIOZ, assigns ratings to the quality of laboratory products (Bioz Stars) based on various factors, including citation index and the impact of articles utilizing the product. Aptum's 2100 Retriever has received a score of 99 out of 100. We extend our gratitude to all the users who have utilized the Retriever and published their remarkable results.
We are confident that other components of the Retriever program, such as the R-Universal epitope recovery buffer and buffers for IHC, will soon gain similar recognition.
Recent Research Using 2100 Retriever :
Andries, V., De Keuckelaere, E., Staes, K., Hochepied, T., Taminau, J., Lemeire, K., Birembaut, P., Berx, G., and van Roy, F. (2019). A new mouse model to study the role of ectopic Nanos3 expression in cancer. BMC Cancer 19, 598.
Bergström, B., Lundqvist, C., Vasileiadis, G.K., Carlsten, H., Ekwall, O., and Ekwall, A.-K.H. (2019). The Rheumatoid Arthritis Risk Gene AIRE Is Induced by Cytokines in Fibroblast-Like Synoviocytes and Augments the Pro-inflammatory Response. Front. Immunol. 10.
Dominy, S.S., Lynch, C., Ermini, F., Benedyk, M., Marczyk, A., Konradi, A., Nguyen, M., Haditsch, U., Raha, D., Griffin, C., et al. (2019). Porphyromonas gingivalis in Alzheimer’s disease brains: Evidence for disease causation and treatment with small-molecule inhibitors. Science Advances 5, eaau3333.
García-Valero, J., Olloquequi, J., Montes, J.F., Rodríguez, E., Martín-Satué, M., Texidó, L., and Ferrer, J.S. (2019). Deficient pulmonary IFN-β expression in COPD patients. PLoS One 14, e0217803–e0217803.
Hummitzsch, K., Hatzirodos, N., Macpherson, A.M., Schwartz, J., Rodgers, R.J., and Irving-Rodgers, H.F. (2019). Transcriptome analyses of ovarian stroma: tunica albuginea, interstitium and theca interna. Reproduction 157, 545–565.
Jung, H.Y., Yoo, D.Y., Nam, S.M., Kim, J.W., Kim, W., Kwon, H.J., Lee, K.Y., Choi, J.H., Kim, D.W., Yoon, Y.S., et al. (2019). Postnatal changes in constitutive cyclooxygenase‑2 expression in the mice hippocampus and its function in synaptic plasticity. Molecular Medicine Reports 19, 1996–2004.
Mancini, G., Pirruccio, K., Yang, X., Blüher, M., Rodeheffer, M., and Horvath, T.L. (2019). Mitofusin 2 in Mature Adipocytes Controls Adiposity and Body Weight. Cell Reports 26, 2849-2858.e4.
McNulty, E., Nalls, A.V., Mellentine, S., Hughes, E., Pulscher, L., Hoover, E.A., and Mathiason, C.K. (2019). Comparison of conventional, amplification and bio-assay detection methods for a chronic wasting disease inoculum pool. PLOS ONE 14, e0216621.
Radcliff, F.J., Waldvogel-Thurlow, S., Clow, F., Mahadevan, M., Johnston, J., Li, G., Proft, T., Douglas, R.G., and Fraser, J.D. (2019). Impact of Superantigen-Producing Bacteria on T Cells from Tonsillar Hyperplasia. Pathogens 8, 90.
Ren, B., Betz, V.M., Thirion, C., Salomon, M., Klar, R.M., Jansson, V., Müller, P.E., and Betz, O.B. (2019). Gene activated adipose tissue fragments as advanced autologous biomaterials for bone regeneration: osteogenic differentiation within the tissue and implications for clinical translation. Scientific Reports 9, 224.
Aptum's collection of Immunohistology buffers offers many benefits, with standardization of your IHC being the foremost advantage. These buffers enable you to achieve the highest quality IHC results without compromising antigen detection. Additionally, the buffers can be utilized in other immune assays, such as immunofluorescence on sections, flow cytometry on fixed cells, and hybridization of sections with antibody detection.
The Retriever IHC buffers empower you to effectively control non-specific staining at every step of the immunohistochemistry process. They are especially recommended for research pathology, where a multitude of polyclonal and/or low-affinity antibodies are commonly used, in contrast to diagnostic applications.
We are delighted to announce the addition of new high-quality tissue slides to our Retriever program, designed to provide optimal section attachment and preserve tissue morphology after epitope recovery in the 2100 Retriever (or any other HIER or proteolytic treatment of tissue sections).
Our developed and produced slides come in two types: supercharged slides, capable of accommodating any tissue type, including small and fatty tissues, and slides that securely bind the tissue through chemical cross-linking.
All of our slides meet the requirements of DIN ISO 8037/1 and are suitable for in vitro diagnostic applications according to IVD Directive 98/79/EC. They are CE-labelled, ensuring their quality and compliance.
Yes, we have achieved it:
You no longer need Tris, EDTA, Citrate, or low or high pH buffers for epitope recovery. Additionally, protease treatment of sections is no longer necessary.
A single R-Universal Buffer can replace all of them!
For certain antibodies, you may still require specific buffers. However, there's no need to worry as we also offer the complete collection of Retriever-adapted analogues of standard recovery buffers.
Recent Publications Using
R-Universal:
Konger, R.L., Derr-Yellin, E., Ermatov, N., Ren, L., and Sahu, R.P. (2019). The PPARγ Agonist Rosiglitazone Suppresses Syngeneic Mouse SCC (Squamous Cell Carcinoma) Tumor Growth through an Immune-Mediated Mechanism. Molecules 24.
Rupp, C., Aakula, A., Isomursu, A., Erickson, A., Kauko, O., Shah, P., Padzik, A., Kaur, A., Li, S.-P., Pokharel, Y.R., et al. (2019). PP2A inhibitor PME-1 suppresses anoikis, and is associated with therapy relapse of PTEN-deficient prostate cancers. BioRxiv 581660.
Störmann, P., Becker, N., Vollrath, J.T., Köhler, K., Janicova, A., Wutzler, S., Hildebrand, F., Marzi, I., and Relja, B. (2019). Early Local Inhibition of Club Cell Protein 16 Following Chest Trauma Reduces Late Sepsis-Induced Acute Lung Injury. Journal of Clinical Medicine 8, 896.
Wagner, N., Dieteren, S., Franz, N., Köhler, K., Perl, M., Marzi, I., and Relja, B. (2019). Alcohol‑induced attenuation of post‑traumatic inflammation is not necessarily liver‑protective following trauma/hemorrhage. International Journal of Molecular Medicine 44, 1127–1138.
Here is how:
For all immunoassays, including ELISA, radioimmunoassay, FLISA, and LTI tests, we provide a comprehensive range of ready-to-use buffers. These buffers are produced following Good Laboratory Practices (GLP) and are certified for diagnostic use.
You can choose from a variety of equally excellent buffers, such as antigen absorption buffers, sample diluent buffers, antibody dilution buffers, and preservation buffers.
Additionally, we offer plate sealers and wash buffers.
We provide everything you need to establish flawless assays in your laboratory or develop reliable and specific commercial tests.
Unique Software from Aptum for Protein Sequence Analysis
Whether you are planning an antibody project, developing a new assay for a pathogen or autoantigen, searching for CTL-epitopes in tumor or viral sequences, or even constructing a new vaccine, you will discover a unique and highly useful program on our website. This program will accelerate your project and provide a solid foundation for success. Please visit www.epiquest.co.uk for more information!