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A reliable, time tested solution to unmask antigen on formalin-fixed sections
Aptum Biologics Ltd.
38 Baker's Drove
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Assay Buffers (ELISA, FLISA and other Assays)
Any measurement of reaction in solid-phase immunoassays contains two components - specific reaction and non-specific reaction.
The value of the results and assay in general greatly depends on whether a non-specific reaction creates false-positive values or not. The presented collection of immunoassay buffers, ISO-certified, for development and production of immunoassay (usually in a plate format) are virtually free from generating a non-specific signal, stable and standardized.
These series of products were designed for academic research groups, using assays to detect the antigen of antibody, and for companies developing commercial diagnostic assays.
See only the specific signal
ISO 9008 certified for diagnostics
Types of Assay
There are several different types of assay you may be working with and developing. Some of them require specific types of reagents (buffers), so here we will establish the terms we will be using when advising on the optimal buffers to be used.
Antigen-coated assay for specific antibodies
This is a standard type of assay aiming at detection in a sample of specific antibodies (directed against the antigen coated on the plate).
Sandwich assay with antibody coating
This is an assay where the presence of antigen in the sample is detected by using a two antibody -Sandwich. A catcher Ab absorbed on the plate binds the antigen from the sample, and the bound antigen is detected by the labelled antibody (tracer).
Competition assay with antibody coating
Competition assay is usually designed for quantitative measurement of the antigen concentration in the sample. The antigen of the sample competes for the binding to the adsorbed catcher antibody with the labelled antigen added to the sample.
Types of Reagents
We offer optimal sets for every step of your assay. For different types of assay and reagents used our sets guarantee the highest quality and standardized result:
Coating Buffers - to absorb the antigen or antibody to the plate
Coating Stabilizers - to protect and preserve the antigen/antibody coated plate for prolonged storage
Plate Blockers - for saturating free binding sites on plates
Assay Diluents - for minimizing non-specific binding, cross-reactivity and matrix effects
Antibody and Conjugate stabilizers - for preserving antibody and conjugate components of the assay
Washing Buffers - for perfect washing of the plate without exposing binding sites anew
Here you can download the full product list and ordering codes
Symbols used in the schemes above:
plate blocking molecules
antibody to antigen measured in the sample
Diluent component blocking biding of the antibody/antigen
Diluent component blocking biding of the labelled antibody/antigen
Labelled antibody ("Tracer") directed against the detected AG or Ab
elisa buffers and reagents
elisa antigen coating
elisa antibody coating
elisa blocking buffer
casein blocking buffer
blocking solution elisa
protein binding surfaces
nonspecific binding cross-reactivities
minimizing nonspecific binding
elisa plate coating buffer
elisa coating buffer
elisa sample dilution buffer
elisa dilution buffer
carbonate buffer elisa
elisa assay buffer
elisa blocking reagent
dilution buffer elisa
elisa binding buffer