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antigen retrieval immunohistochemistry, formalin fixed paraffin embedded, immunohistochemistry protocol, antigen recovery, antigen retrieval buffer, tissue sections, antigen unmasking, antigen retrieval protocol, antigen retrieval pressure cooker, antigen retrieval microwave, antigen retrieval methods, epitope retrieval, antibody, tissue section, immunohistochemistry, paraffin, formalin, citrate buffer, pathology, ihc
A reliable, time tested solution to unmask antigen on formalin-fixed sections
Aptum Biologics Ltd.
38 Baker's Drove
Southampton
SO16 8AD
United Kingdom
...all buffers are produced at GLP conditions and are certified for use in diagnostics...
Making your IHC standardized and reproducible
These are several products to help you achive even better results with your immundetection of epitopes.
One for all: Universal Buffer for Epitope Recovery
New, patent-pending R-Universal buffer for epitope recovery on routinely fixed tissue material. When used in Retriever, it removes cross-links created by paraformaldehyde fixative, eliminates precipitated bivalent cations, and allows perfect refolding of damaged epitopes. It can be used with any antibody working in western blot, and many others. Replaces High, Low, etc buffers. Certified for diagnostics.
New Technology of Blocking Buffers for Improved Specificity
Most blocking buffers used in various solid-phase applications, such as IHC, Western blot, or ELISA, typically rely on proteins, often with charged properties, to bind to sites capable of non-specific protein binding. This binding prevents subsequent non-specific binding of antibodies. However, this approach presents two main drawbacks: 1) certain antibodies can interact with the blocking proteins themselves, and 2) the blocking proteins may obstruct the necessary epitopes due to steric hindrance. Moreover, blocking proteins can create locally positively charged areas that might attract negatively charged components of the assay, such as antibodies or conjugates.
Our technology, employed in all our immunoassay buffers, overcomes these issues, allowing you to circumvent these problems. This ensures effective reduction of background signals without any undesired side effects.
Immunoassay buffers: Improve Specificity/Sensitivity
Based on the proposed novel technology, we created buffers to prevent non-specific binding of either antibodies or conjugates. Buffers include sample (or primary antibody) diluent and blocking buffer.
The set of buffers for each type of application also includes additional buffers for washing, stripping (blot), peroxidase stabilisers and diluents. All buffers are ISO certified for diagnostics applications.
Colour & Pattern Coding of Buffers
All buffers are pattern and colour coded, this makes them easy to distinguish in the refrigerator, and not to mix using different sets for different applications.
Arrays: Stain All Tissue Samples in One Go
We offer a unique, handy manual arrayer and pre-made recipient blocks. Anyone can easily make their own arrays, thus placing pre-selected areas of tissue for staining them all on one slide.