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A reliable, time tested solution to unmask antigen on formalin-fixed sections
Aptum Biologics Ltd.
38 Baker's Drove
Retriever-Grade High Adhesivity Tissue Slides
ISO 8037/1 standard
Certified for diagnostics
As both antigen recovery and in situ hybridization are quite aggressive methods, the quality of the tissue slide that holds the section is critical for the outcome being excellent. Sometimes we had to resolve issues with a tissue detachment in experiments performed using Retriever, and the reason has been always the same: poor quality tissue slides (which quite saturated the market).
To address this issue, we offer new types of tissue slides for heat-induced epitope recovery in Retriever (or any other HIER system). The slides carry the trademark Histobond®, as they were developed together with Marienfield GmbH, one of the leading slides producers, and are produced in Germany to the highest industry standards.
The slides were primarily designed to resolve all the problems for Retriever users and other researchers performing IHC or IF on FF/PE sections. In particular, the following issues:
Tissue detachment in EDTA/alkaline buffers
Issues with prolonged incubation with diluted Ab during immunostaining
High stringency washing during immunostaining
Detachment of small size tissues (such as array tissue, embryo tissue, other tiny samples)
Detachment of narrow samples (such as mouse skin sections)
Poor adhesion or fatty tissues, including brain tissue, MA Mary grand tissue, adipose tissue
Adhesion of plastic-embedded tissue
Issues with heating and high-stringency washing during in-situ hybridization.
Tissue slides with superior charged surface, consistency of lots, and best physical characteristics of glass used,
Recommended for all impirtant experiments, experimental pathology, IHC and IF.
Histobond+X, Covalent Binding
Superior quality slides with coating covalently binding the tissue. Use for all small tissue section (or small at least in one dimention). Surerior for fat tissues, arrays, and similar experiments with valuable materials.
Histobond+F, Routine & Cryostat
High quality tissue slidesfor cryostat tissues and routine IHC staining for pathology and histology experiments.
General Technical Characteristics
the slides are made of soda-lime glass of third hydrolytic class. This glass fulfils all relevant optical requirements for light microscopy;
meet the requirements of DIN ISO 8037/1;
dimensions: approx. 76 x 26 mm;
thickness: approx. 1 mm (tol. ± 0.05 mm);
pre-cleaned and ready for use. Our slides are cleaned thoroughly by multi-stage processes without using any surfactants. Thus we accomplish ready for use, pre-cleaned, well wettable slides‘ surfaces;
finely ground edges with 90º shape. We apply water-cooled grinding processes which results in remarkably smooth and burr-free edges.
have an imprinted frosted end of approx. 20 mm, which can be printed with various printer systems and labelled with permanent markers. Dark markings contrast, especially well with the bright colours of the labelling areas and thus facilitate the identification of preparations. The thin layer of the labelling area prevents the slides from sticking together, which facilitates the use of these slides on automated machinery.
supplied in plastic boxes of 100 pieces, 20 boxes in a carton
Microscope slides for in vitro diagnostic applications are manufactured acc. to ISO 8037/1 standard
Tissue slides meet the requirements of DIN ISO 8037/1
for in vitro diagnostic applications according to IVD Directive 98/79/EC, CE-labelled, with recommended best- before date and batch number for comprehensive information and traceability