Epitope recovery for Phospho-Proteins

Unlock Reliable Phospho-Protein Recovery with Aptum’s R-Universal Buffer

When it comes to antigen retrieval, especially for sensitive post-translational modifications like phosphorylation, Aptum’s R-Universal buffer delivers exceptional results. Trusted by researchers worldwide, R-Universal buffer is specifically optimized to preserve the integrity of phosphorylated epitopes, making it the go-to choice for phospho-specific antibody staining in immunohistochemistry and immunofluorescence.

Proven effective in studies targeting TGF-β signaling, MAPK pathways, synaptic proteins, and disease-related kinases—including those linked to cancer, neurodegeneration, and organ dysfunction—R-Universal buffer consistently enhances staining quality and reproducibility.

Image from: Patel, V.N.et al., 2024. Specific 3-O-sulfated heparan sulfate domains regulate salivary gland basement membrane metabolism and epithelial differentiation. Nat Commun 15, 7584. https://doi.org/10.1038/s41467-024-51862-0

Explore our curated collection of peer-reviewed articles showcasing how R-Universal buffer has enabled precise, reliable detection of phosphorylated proteins across a wide range of biomedical research applications. Discover the difference R-Universal buffer can make in your phospho-protein studies!

Noskovičová et al. (2018)CDCP1 negatively regulates TGF-β signaling and myofibroblast differentiation→ Used R-Universal buffer for treating lung sections prior to phospho-Smad3 staining.Link

Ostrosky-Frid et al. (2021)Role of KLHL3 and dietary K⁺ in regulating KS-WNK1 expression→ SPAK and phospho-SPAK antibodies used post-R-universal buffer treatment.PDF

Ugidos et al. (2019)ANKRD55 interactome analysis→ Applied R-Universal buffer before phospho-specific antibody staining in neuroimmune pathways.PDF

Patel et al. (2024)Heparan sulfate and epithelial differentiation in salivary glands→ R-Universal buffer enabled visualization of phospho-ERK.PDF

Zhang et al. (2015)SPAK kinase domain essential for blood pressure control→ Kidney tissue treated with R-universal buffer followed by phospho-protein staining.PDF

Jensen et al. (2024)Detection of early alpha-synuclein pathology with MJF-14 PLA→ Staining for phosphorylated S129 α-synuclein post-buffer treatment.Link

Perez-Corredor et al. (2024)APOE3 Christchurch modulates Wnt signaling in Alzheimer's→ Used R-universal buffer in phospho-β-catenin detection.PDF

Siew et al. (2024)Integrated analysis of spaceflight-induced kidney dysfunction→ R-Universal used before phospho-specific antibody staining.PDF

The advantage of using R-Universal is that it typically allows for multiple antibody stainings after just a single round of epitope retrieval. Most epitopes can be successfully recovered using the 2100 Antigen Retriever with R-Universal, while also ensuring efficient recovery of phospho-epitopes.